منابع مشابه
Analysis of cell cycle by flow cytometry.
Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4',6'-diamidino-2-phenylindole (DAPI) and deconvolution of the cellular DNA content frequency histograms. This approach reveals distribution of cells in three major phases of the...
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Perturbation of the tubulin/microtubule dynamic in cells is perhaps the single most important mode of action of anticancer drugs. Standard methods for identifying and evaluating compounds for their ability to alter tubulin polymerization are low throughput, labor intensive, expensive, or make their assessment in vitro. Here we report a method to rapidly quantify the extent of tubulin polymeriza...
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T cells are an essential cellular component of the immune system. When T cells encounter antigen and receive co-stimulatory signals, they become activated, proliferate and produce cytokines. Flow cytometry is a valuable research tool in analyzing T cell functions including proliferation, survival and cytotoxicity as well as cytokine production and cell signaling. This article has reviewed the u...
متن کاملAutomation of human sperm cell analysis by flow cytometry.
Semen sample analysis is routinely performed by microscopical evaluation and manual techniques by laboratory operators; the analysis is affected by a wide imprecision related to variability among observers, influencing its clinical validity. Our aim was to automate sperm analysis with the use of flow cytometry for evaluation of cell counts and typing and with the use of a new membrane-permeant ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2001
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20010801)44:4<369::aid-cyto1129>3.0.co;2-d